LOCUS       IS26-mef(B)-sul3-IS440_unit5998 bp   DNA   linear   BCT 03-OCT-2020
DEFINITION  Escherichia coli.
ACCESSION   
VERSION
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; gamma subdivision; Enterobacteriaceae;
            Escherichia.
REFERENCE   1  (bases 1 to 5998)
  AUTHORS   Zhou,D.
  TITLE     Complete sequence of IS26-mef(B)-sul3-IS440 unit
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5998)
  AUTHORS   Zhou,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-OCT-2020) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Microbiology and Epidemiology,
            Beijing, Beijing 100071, China
FEATURES             Location/Qualifiers
     source          1..5998
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:562"
     repeat_region   1..14
                     /gene="IRL_IS26"
     CDS             64..768
                     /gene="tnpA"
                     /codon_start=1
                     /transl_table=11
                     /product="IS26 transposase TnpA"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDV
                     EHRQIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYY
                     GDPLGEMRLVSRVFEM"
     repeat_region   807..820
                     /gene="IRR_IS26"
     CDS             1172..2401
                     /gene="mef(B)"
                     /codon_start=1
                     /transl_table=11
                     /product="Macrolide efflux determinant"
                     /translation="MNRIKNWKKQFAVIYTGQAFSILGSAAVQFAVIWWLTIQTESAI
                     TLTIASLVAFLPNMLIGPFAGVWIDRYNRRTVMILADGLVAVSSIILGAAFLLVETPP
                     IWFIYIVLFLRGLGNTFHGPAMQAAIPMFVPADMLTKAGGWGNMIQSISNMMGPVLGA
                     ALMSFLPISSIMIVDILGAAFAIVCLLFVIIPDITQTNEKMSVLSDMKQGFIAMKANK
                     PLMAVFSPMLLMTILYMPLGSLFPLLARSHFMGEAWHNSIVEFVFAGGLLLSSLVIGV
                     WGGMKRRFFMASLAIGLMGLATLISGALPTSGFWIFVICCFFLGASGTFMNVPVMAYV
                     QESIAPEMMGKVFSLLMTAMTLSMPIGLLVAGPVVEVIGVNTWFFWSGVALIVNAVLC
                     RILTRRYDKVTMKPQVD"
     CDS             2547..3410
                     /gene="yusZ"
                     /codon_start=1
                     /transl_table=11
                     /product="Short chain dehydrogenase"
                     /translation="MIPNSENKRVWFITGASKGLGYAFTCAALKAGDKVVAVARTIDN
                     LAKLEETYQESLLPLNLDVTDREAVFSTVETAVKHFGRLDIVVNNAGIMTMGMIEELN
                     ESDARKLMDTNFFGALWVCQAVMPYLRSQRSGHIIQITSIGAIISGPMSGIYSASKFA
                     LEGMSEALAKEAEHFGVKLTMVEPGGYWTDLYTSMSYSNPLDSYGTLRDELAKQYSED
                     SVDSDPSLAAEALMKLVASNNPPLRLILGSMVYDLAMDTLKARMATWEEWEAVSRASE
                     KAIPAPERYGV"
     CDS             3448..3693
                     /codon_start=1
                     /transl_table=11
                     /product="Hypothetical protein"
                     /translation="MKIEIMEYNPDWTKNFEEEKIKLLHFFGSHAVAIEHIGSTAIPN
                     QRAKPVIDIFIGVSPFAELPFISAFLMQRSITTLRQI"
     CDS             3823..4071
                     /codon_start=1
                     /transl_table=11
                     /product="Hypothetical protein"
                     /translation="MASWGSYVFELCRINANQGLTAEIDWNYTSWSRRCRKILIGLVV
                     RGAKTVVVRGKNRILTQNREKLEMTVPISFHNELKQKN"
     CDS             4162..4953
                     /gene="sul3"
                     /codon_start=1
                     /transl_table=11
                     /product="Dihydropteroate synthase"
                     /translation="MSKIFGIVNITTDSFSDGGLYLDTDKAIEHALHLVEDGADVIDL
                     GAASSNPDTTEVGVVEEIKRLKPVIKALKEKGISISVDTFKPEVQSFCIEQKVDFIND
                     IQGFPYPEIYSGLAKSDCKLVLMHSVQRIGAATKVETNPEEVFTSMMEFFKERIAALV
                     EAGVKRERIILDPGMGFFLGSNPETSILVLKRFPEIQEAFNLQVMIAVSRKSFLGKIT
                     GTDVKSRLAPTLAAEMYAYKKGADYLRTHDVKSLSDALKISKALG"
     repeat_region   5002..5018
                     /gene="IRL_IS440"
     CDS             5200..5886
                     /gene="tnpA"
                     /codon_start=1
                     /transl_table=11
                     /product="IS440 transposase tnpA"
                     /translation="MLVNPNNPIFNLATGKVLLKVPQVRGMEFYPSCIEKGMRSERAL
                     KLAIAEMYVKGVSTRRVSDIVEILCGTEVSSSQVSRLAKELDEEITSWKAQPVGQIQY
                     LVLDATYESVRVGSHVVKQALLVAIGVDYSGNRHILDAEVANSEAEVNWRSFLEGLVR
                     RGMHGLRMITSDDHSGLRAAIDAVFPGILWQRCQFHLQQNAHSYVTKKDEIPLIAADI
                     RKVFNRNMSR"
     repeat_region   5982..5998
                     /gene="IRR_IS440"
ORIGIN      
        1 ggcactgttg caaatagtcg gtggtgataa acttatcatc cccttttgct gatggagctg
       61 cacatgaacc cattcaaagg ccggcatttt cagcgtgaca tcattctgtg ggccgtacgc
      121 tggtactgca aatacggcat cagttaccgt gagctgcagg agatgctggc tgaacgcgga
      181 gtgaatgtcg atcactccac gatttaccgc tgggttcagc gttatgcgcc tgaaatggaa
      241 aaacggctgc gctggtactg gcgtaaccct tccgatcttt gcccgtggca catggatgaa
      301 acctacgtga aggtcaatgg ccgctgggcg tatctgtacc gggccgtcga cagccggggc
      361 cgcactgtcg atttttatct ctcctcccgt cgtaacagca aagctgcata ccggtttctg
      421 ggtaaaatcc tcaacaacgt gaagaagtgg cagatcccgc gattcatcaa cacggataaa
      481 gcgcccgcct atggtcgcgc gcttgctctg ctcaaacgcg aaggccggtg cccgtctgac
      541 gttgaacacc gacagattaa gtaccggaac aacgtgattg aatgcgatca tggcaaactg
      601 aaacggataa tcggcgccac gctgggattt aaatccatga agacggctta cgccaccatc
      661 aaaggtattg aggtgatgcg tgcactacgc aaaggccagg cctcagcatt ttattatggt
      721 gatcccctgg gcgaaatgcg cctggtaagc agagtttttg aaatgtaagg cctttgaata
      781 agacaaaagg ctgcctcatc gctaactttg caacagtgcc attttgaccc aaaaccgcga
      841 aaaactcgaa atgacagttc ctatcagttg aagtgtaatg caaaatattc aggaaaagag
      901 ggtcggcatt cgtagtacgc tgtcgttctt gaaattacac taaaaacgtg ctacaataaa
      961 aaaattgaag cggggtagaa atatgtattt gatattcatg taatttagtt tttaagcact
     1021 gcccttttaa gggtttattt gttgtgctta aagacatatt tacatgagta tatcctcgct
     1081 tttgaagacc tccttgtaaa attatgtctt tctgcacgaa caacgcttag gcagttgttc
     1141 gtttttaatt ttatataagg agatttctat aatgaacaga ataaaaaatt ggaagaaaca
     1201 atttgctgta atatacacag ggcaggcttt ttcaatcttg ggttctgccg cagtgcagtt
     1261 cgctgttatc tggtggctga ccatccagac tgaatccgca atcaccttga cgattgcatc
     1321 cttagttgcc tttctcccca atatgttaat cggacccttt gccggtgtgt ggatcgaccg
     1381 atacaaccgc cgaacagtaa tgattttagc tgacggtctg gtagctgtat ccagcatcat
     1441 ccttggggca gcatttttac ttgtggaaac accccctatt tggtttatct acattgtttt
     1501 atttttgcgt ggattgggga atacctttca cggtccagct atgcaagcgg cgatacccat
     1561 gtttgtgcca gcagatatgt tgaccaaagc agggggctgg ggaaatatga tccaatcaat
     1621 atccaacatg atggggcctg tgctgggtgc tgcgcttatg tcatttctac ctatttcctc
     1681 cattatgatt gtggatatac tgggagccgc ttttgcgata gtttgcctcc tatttgttat
     1741 aattccagac attacgcaaa ccaatgagaa gatgagtgta ttgtctgaca tgaagcaggg
     1801 ctttatcgca atgaaagcaa ataaaccttt aatggctgtg ttttccccca tgctgctgat
     1861 gaccatactt tatatgccat taggttctct gttccctcta ctggcacgca gccactttat
     1921 gggtgaagcc tggcacaata gcattgtgga atttgtcttt gcaggtggat tgcttctttc
     1981 atctttggtt atcggtgtat ggggcggcat gaaaagaagg tttttcatgg catccttagc
     2041 tattggctta atgggtctgg ctacactgat tagcggagcg ctaccgacaa gcggtttttg
     2101 gatatttgtt atatgctgct tcttcttggg cgcctctggc acatttatga atgttcctgt
     2161 tatggcttat gttcaagaaa gcattgcccc tgaaatgatg ggcaaggtgt tttccctttt
     2221 gatgaccgcc atgactcttt ctatgccgat aggcttactt gttgcaggtc cggttgttga
     2281 ggttataggt gttaatacat ggtttttctg gtctggtgtt gcgttgatag taaacgctgt
     2341 tctctgccgc attctgacac gacgctatga caaagtaaca atgaaaccgc aagtggactg
     2401 aaaaaaggac cgggttgatg ataatttgta gtggtgagct tctgggagta caaaacaaag
     2461 tgctcaaaat tgtcgggctc atggcgttta acggtattaa tttcgcttat aataatcttt
     2521 ctataatagc ctaaaggaga atatctatga tacctaatag cgaaaataaa agagtatggt
     2581 ttattaccgg agcaagcaag gggcttggct atgcttttac atgcgccgcc ttgaaagccg
     2641 gggataaagt tgttgcagtt gcaaggacta tcgataattt ggcgaagcta gaagaaacat
     2701 atcaagagag cttactgcca ttaaacctcg atgttacaga tagggaggct gttttttcta
     2761 cggttgaaac agcagttaaa catttcggta ggcttgatat tgttgttaat aatgcgggta
     2821 tcatgactat gggtatgatt gaagaactaa acgaatccga tgctcggaaa ctaatggaca
     2881 caaacttttt tggagctctt tgggtttgtc aggcagtgat gccctatttg aggtcgcagc
     2941 gttctggaca tatcatacag attacaagca ttggcgctat tatctcgggt ccgatgtctg
     3001 gtatttacag tgcaagtaaa tttgcgctgg aaggaatgag tgaagcctta gcaaaagaag
     3061 cggaacattt cggagtgaaa ctcactatgg tggaaccagg aggatattgg acagatttat
     3121 acacttcgat gagttacagc aatccattag attcatacgg tacactgcgc gatgagctgg
     3181 cgaaacagta ttccgaagat tcggtcgata gtgatccttc cttggcagcg gaagccctta
     3241 tgaaattagt agccagcaat aatccgcctt tgaggcttat ccttggcagc atggtatacg
     3301 atttggcgat ggatacctta aaagcacgga tggctacctg ggaagaatgg gaagctgtta
     3361 gccgtgcatc agaaaaggct attcctgcac cggagagata tggagtataa taccaaacat
     3421 ggtttttgag gcaaggacga aactatcatg aaaattgaaa ttatggaata taacccggat
     3481 tggacaaaga attttgaaga agagaaaata aaattgctcc attttttcgg ttctcatgcc
     3541 gtagccattg aacatatcgg aagcacggcg atacccaatc aacgggcgaa accggttata
     3601 gatatattta ttggcgtttc gccttttgct gaactacctt ttatcagcgc atttttaatg
     3661 caaaggagta tcaccacact ccgacagata tgacaggcag atatttgttt gcaaaatata
     3721 caaatgaagt ttggacccat aatcttcttg ctttggtgta ggtataatac ccaaaaacga
     3781 agtttatgcg gaacaggata acacctcgaa atcggagttt atatggcttc gtgggggagt
     3841 tatgtgtttg agctttgtcg cattaacgcg aatcaggggt tgacggcaga aatagactgg
     3901 aattacactt cttggagtcg gcgttgccgg aaaattctga ttggattagt tgttcggggt
     3961 gcgaaaacag tcgtagttcg gggaaaaaac cgaattttga cccaaaaccg cgaaaaactc
     4021 gaaatgacag ttcctatcag ttttcataat gaattaaaac aaaaaaatta ggctgcaaag
     4081 atagggaaaa tgtgtcaatt tgcgtaagtg ataattagtc aattaagata aatgctactt
     4141 ttgtaatcaa agcaaaatga tatgagcaag atttttggaa tcgtaaatat aaccaccgat
     4201 agtttttccg atggaggact ttatttagat acagataagg caattgagca tgctctgcat
     4261 ttggttgaag atggagcaga tgtgattgat ttgggagccg cttccagtaa tcctgataca
     4321 actgaagtgg gcgttgtgga agaaatcaaa agactcaaac ctgtcattaa ggctttaaaa
     4381 gaaaaaggca tttctatttc tgttgataca tttaaacctg aggttcagag tttttgcata
     4441 gaacaaaagg ttgattttat taatgatatt caaggttttc cttatcctga gatttattca
     4501 ggcttggcaa agtcagattg caaacttgtg ttgatgcact ccgttcagcg aattggtgca
     4561 gctactaaag ttgaaacgaa tccggaagag gtttttactt ccatgatgga attttttaaa
     4621 gaaagaattg ctgctttagt tgaggctggt gtaaagcgtg aacgaattat tcttgatccg
     4681 ggtatgggct tctttttagg ctctaatcca gaaacatcta ttcttgtttt gaagcgtttc
     4741 cctgaaattc aagaagcttt taatttgcaa gtaatgattg cagtgtcacg gaaatcattc
     4801 ttaggtaaaa taactggaac cgatgtgaaa tctcgtttag caccaactct tgcagcagaa
     4861 atgtatgcat acaaaaaagg tgcagattat ctccgcaccc atgatgttaa gtctttatca
     4921 gatgccttga aaatatccaa agccctaggt tagttttatt tttttagctt tctaaatgaa
     4981 tatgctatac ttgctctgaa gttgtgttgg cgtacttgac ttaggtctgg atctttcgaa
     5041 ctgttactaa gagggtaatc atagccaaac ataaactgga aatgtcctaa ttctaaacct
     5101 gttccaatac ctacactata atcaagagga cttaaacttc catcacttcc aaaaggtttt
     5161 gtgtacttct cttgagtttt cgtagctaaa tagtagtgtt tgttggtaaa tccaaataat
     5221 ccaatattca atcttgcgac cgggaaagtc ctcttgaaag taccacaagt tcgcgggatg
     5281 gagttttatc ccagctgcat cgagaaaggc atgcgcagtg agcgtgctct caagctcgcc
     5341 atcgccgaaa tgtatgtcaa aggagtaagt acccgcaggg tctcggatat cgtcgaaatt
     5401 ctttgtggca ccgaagtcag ctcgtcccag gtcagcaggc tggcaaagga gctcgatgaa
     5461 gagattacgt cttggaaggc gcagcctgtc ggacagattc aatacttggt acttgatgcg
     5521 acctatgaat cggttcgcgt cggttcccat gtggtcaagc aggcgcttct agtggctatt
     5581 ggcgttgatt acagcgggaa tcggcatatt cttgacgccg aagtcgcgaa cagtgaggca
     5641 gaggtaaact ggcgttcctt tctcgagggt ctcgtacgac gagggatgca cggcctgcga
     5701 atgatcacca gtgatgacca ctcaggactg cgcgcggcaa tcgatgctgt cttccctgga
     5761 attctgtggc aacgctgcca gtttcatctg cagcagaatg cccactccta cgtcacgaaa
     5821 aaagatgaga tcccgctgat agccgctgat attcggaagg tgttcaatcg gaatatgtca
     5881 agatagttta tgccagattt ttgagaggtt tgcatcaagc agccttcgaa agaggacggc
     5941 atgacattga gaccggcaag gcgtcgtaga tacgcacttt cccagtacgc caccgtaa
//
